On Sun, Nov 27, 2011 at 8:45 PM, Choons <chadkimrey@gmail.com> wrote:
us and may be overpriced or hard to get. I'm thinking primarily about
wet products like enzymes, media, buffers, cofactors, etc. as opposed
to lab equipment.
here's a list i keep tucked away...
* Boric acid
* EDTA
* Tris base
* Sodium chloride
* Sodium hydroxide
* Sodium acetate
* Potassium chloride
* Potassium acetate
* Bleach (diluted to a final concentration of 1.5% sodium hypochlorite)
* Hydrochloric acid
* Glacial acetic acid
* Triton® X-100
* Glycogen
* Glycerol
* Gelatin
* Sodium sulfate
* Sodium iodide
* Silica particles
* Nitric acid
* Chelex® 100 (Bio-Rad Laboratories, Richmond, CA, No. 143-2832)
* Proteinase K
* Potassium phosphate
* Disodium phosphate
* Sodium dodecyl sulfate (SDS)
* Guanidine isothiocyanate
* Sodium citrate
* β-mercaptoethanol
* Sarkosyl
* Saponin
* tRNA (yeast or E. coli)
* Phenol (equilibrated with water for RNA and equilibrated with Tris
pH 8.0 for DNA)
* Isoamyl alcohol
* Chloroform
* Isopropanol
* 100% ethanol
* Peptone
* Tryptone
* Yeast extract
* Agar
* Agarose
* Ethidium bromide
* Bromophenol blue
* Xylene cyanol
* Tryptic Soy Agar (V. cholerae)
* Barium chloride dihydrate for MacFarland's Standard (V. cholerae)
* Sulfuric acid for MacFarland's Standard (V. cholerae)
* Saponin (P. falciparum/P. vivax)
* N-acetyl cysteine (M. tuberculosis)
* Sterile phosphate buffered saline (PBS)
* Sodium thioglycolate for Cary-Blair transport medium (E. coli)
* Calcium chloride for Cary-Blair transport medium (E. coli)
* MacConkey agar (E. coli)
* Acetone (Leptospira)
* Sterile Dacron®-tipped applicators (Fisherbrand #14-959-90) (C.
trachomatis/N. gonorrhoeae)
* Nucleotides (dNTPs: dATP, dCTP, dGTP, dTTP)
* 10X Buffers
* MgCl2
* Taq polymerase (see section 5.1.2)
* Reverse transcriptase RAV-2 (Amersham Corp., Arlington Heights, IL)
* rTth reverse transcriptase-polymerase (Perkin-Elmer Corp.)
* Dithiothreitol (DTT)
* Dimethyl sulfoxide (DMSO)
* Tetramethylammonium chloride (TMAC)
* Betaine (Sigma Chemical Co., St. Louis, MO, B-2629)
* Bovine serum albumin (BSA)
* Manganese acetate
* Bicine
* Nuclease-free sterile ddH20
* Mineral oil
* Wax beads
* Ampicillin
* Potassium acetate
* Glucose
* Polyethylene glycol (MW 8000)
* Polyethylene glycol (MW 3350)
* Magnesium sulfate
* 5-Bromo-4-chloro-3-indolyl-D-galactopyranoside (X-Gal)
* Isopropyl β-D-thiogalactopyranoside (IPTG)
* Dimethyl formamide (DMF)
* T4 DNA Ligase
* 10X T4 DNA Ligase Buffer
* RNase
http://heybryan.org/
1 512 203 0507
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