On Wed, Nov 23, 2011 at 2:25 PM, Simon Quellen Field <sfield@scitoys.com> wrote:
> For DNA quantification, a simple and reliable way to get self-calibration
> is to use three optical paths.
> One goes through a sample of DNA at a known concentration higher than
> what you want or expect the sample to be.
> A second goes through a sample of DNA at a known concentration lower
In my 4 years studying biotech, I've never seen a sample of known
concentration being sold from a company... lyophilized DNA, yes, but
then you have to trust your pipet's calibration... and at the volumes
we work with evaporation will change concentrations within minutes if
something is left uncapped.
> than what you want or expect the sample to be.
> The third goes through the sample.
> The Beer-Lambert law will be linear between the two samples of known
> concentration.
> The sample will lie between those two known concentrations, and can be
> linearly
> interpolated easily. This can get you much higher accuracy than looking up
> molar
> absorptivities in a table and multiplying. And it is dead simple for an
> Arduino to do.
>
>
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>
>
>
> On Wed, Nov 23, 2011 at 9:07 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
>>
>> On Wed, Nov 23, 2011 at 2:55 AM, Robert O'Callahan <ropoctl@rice.edu>
>> wrote:
>> > I've only been casually skimming this open source spec thread -- are
>> > you guys planning to make a nanodrop version of that? I would gladly
>> > pay $1000 for a similar product.
>>
>> I'm aiming for DNA quantification functionality. It should still be
>> less than $1000
>>
>> >
>> > On Tue, Nov 22, 2011 at 11:48 PM, Nathan McCorkle <nmz787@gmail.com>
>> > wrote:
>> >> I've been straying away from the idea of a monochromator because it
>> >> has moving parts, but maybe it could be ruggedized with the right
>> >> components. The reason I bring this up is the auto-calibration enabled
>> >> by a 2D sensor... makes driving the stepper/servo a bit easier on the
>> >> motor control side, right?
>> >>
>> >> TI makes those digital micromirrors... we built a dev kit for one at
>> >> work at about 6-12 inches working depth it had a sweep of a good 6
>> >> inches up and down. I'm not sure if they sell a chip with just one
>> >> mirror though, and I think the coating might be UV absorbent.
>> >>
>> >>
>> >> http://www.ti.com/general/docs/datasheetdiagram.tsp?diagramId=321&genericPartNumber=DLP5500&isFunctional=Y&isFunctional=Y
>> >>
>> >> Not sure how it could help, but here's an example the 2D mirrors in
>> >> projector mode, and in single-pixel camera mode:
>> >> http://www.ti.com/lit/ml/sprp592/sprp592.pdf
>> >>
>> >> On Tue, Nov 22, 2011 at 10:12 PM, John Griessen
>> >> <john@industromatic.com> wrote:
>> >>> On 11/22/2011 08:25 PM, Nathan McCorkle wrote:
>> >>>>>
>> >>>>> Use two fibers.
>> >>>>
>> >>>> one bypassing the sample?
>> >>>>
>> >>>
>> >>>
>> >>> Sure. You can glue fibers into a mold shape, then cut and polish
>> >>> so they have exact spatial relations. Surgeons have long used
>> >>> light pipes made that way.
>> >>>
>> >>> JG
>> >>>
>> >>> PS ways to use fiber optics is more on topic.
>> >>> electronics design details isn't.
>> >>>
>> >>> --
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>> >>
>> >>
>> >>
>> >> --
>> >> Nathan McCorkle
>> >> Rochester Institute of Technology
>> >> College of Science, Biotechnology/Bioinformatics
>> >>
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>>
>> --
>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
>>
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