For DNA quantification, a simple and reliable way to get self-calibration
is to use three optical paths.
One goes through a sample of DNA at a known concentration higher than
what you want or expect the sample to be.
A second goes through a sample of DNA at a known concentration lower
than what you want or expect the sample to be.
The third goes through the sample.
The Beer-Lambert law will be linear between the two samples of known concentration.
The sample will lie between those two known concentrations, and can be linearly
interpolated easily. This can get you much higher accuracy than looking up molar
absorptivities in a table and multiplying. And it is dead simple for an Arduino to do.
-----
Get a free science project every week! "http://scitoys.com/newsletter.html"On Wed, Nov 23, 2011 at 9:07 AM, Nathan McCorkle <nmz787@gmail.com> wrote:
On Wed, Nov 23, 2011 at 2:55 AM, Robert O'Callahan <ropoctl@rice.edu> wrote:I'm aiming for DNA quantification functionality. It should still be
> I've only been casually skimming this open source spec thread -- are
> you guys planning to make a nanodrop version of that? I would gladly
> pay $1000 for a similar product.
less than $1000
>
> On Tue, Nov 22, 2011 at 11:48 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>> I've been straying away from the idea of a monochromator because it
>> has moving parts, but maybe it could be ruggedized with the right
>> components. The reason I bring this up is the auto-calibration enabled
>> by a 2D sensor... makes driving the stepper/servo a bit easier on the
>> motor control side, right?
>>
>> TI makes those digital micromirrors... we built a dev kit for one at
>> work at about 6-12 inches working depth it had a sweep of a good 6
>> inches up and down. I'm not sure if they sell a chip with just one
>> mirror though, and I think the coating might be UV absorbent.
>>
>> http://www.ti.com/general/docs/datasheetdiagram.tsp?diagramId=321&genericPartNumber=DLP5500&isFunctional=Y&isFunctional=Y
>>
>> Not sure how it could help, but here's an example the 2D mirrors in
>> projector mode, and in single-pixel camera mode:
>> http://www.ti.com/lit/ml/sprp592/sprp592.pdf
>>
>> On Tue, Nov 22, 2011 at 10:12 PM, John Griessen <john@industromatic.com> wrote:
>>> On 11/22/2011 08:25 PM, Nathan McCorkle wrote:
>>>>>
>>>>> Use two fibers.
>>>>
>>>> one bypassing the sample?
>>>>
>>>
>>>
>>> Sure. You can glue fibers into a mold shape, then cut and polish
>>> so they have exact spatial relations. Surgeons have long used
>>> light pipes made that way.
>>>
>>> JG
>>>
>>> PS ways to use fiber optics is more on topic.
>>> electronics design details isn't.
>>>
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>>>
>>
>>
>>
>> --
>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
>>
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>>
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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