Re: 16SRNA sequencing to determine differences between bacteria species

(Specifically Patrik's response):
On Oct 6, 12:00 am, Nathan McCorkle <nmz...@gmail.com> wrote:
> I was thinking that colony PCR is really quite easy, are there
> universal primers for 16S? I could get some in the mail in a few days
> for not more than $5-$15... not sure if I really need that level
> though, since I think this Prof is a "very very fair" grader.

JGI typically uses universal primers 27F/1391R for bacterial 16S rRNA:
http://my.jgi.doe.gov/general/protocols/SOP_16S18S_rRNA_PCR_Library_C...
The Wikipedia page actually has a table with universal primers as
well:
http://en.wikipedia.org/wiki/16S_ribosomal_RNA#Universal_Primers
16S is such a common target in microbiology, that chances are somebody
at your lab may have some primers available that you could use. Ask
around among the grad students and postdocs...

On Thu, Dec 1, 2011 at 11:15 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> See this recent thread, you'll need lab access and tools to perform PCR:
>
> How to perform bacterial ID (I am trying to determine a Bacillus species)
> http://groups.google.com/group/diybio/browse_thread/thread/449334a66c22444a
>
> On Thu, Dec 1, 2011 at 9:58 PM, Koz <kozovski.a@gmail.com> wrote:
>> Hi ,
>>
>> I want to do a fairly simple test and determine specific species of
>> bacteria that exist on different surfaces.
>> The agar plate/phenotype test does not give me enough precise data, so
>> I was wondering if there is a relatively inexpensive way to sequence
>> microorganisms from a collection of swabs?
>> Pretty new to this and have very limited access to basic lab equipment
>> so supposedly I would need to send this out.
>> Any suggestions?
>>
>> Alexander
>>
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>
>
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics
>

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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