Re: Get a plasmide - buy? share?

Wow.
Have a look at that: http://www.youtube.com/watch?v=TZeL7WIkQC0
He just uses a dish detergent and sodiumhydroxide (both of which I
already have at home) and isopropanol to conduct an alkaline -home-
miniprep!!

I immagine that the purity of the plasmids is not very high, maybe
some 80-85% ??

But anyway, is it neccessary for a transformaiton to have relatively
pure plasmids? Some proteins and other substances (e.g. luciferin,
sugar, etc.) that are in the solution don't affect the would-be
transformed bacteria, thay may consume them or not. No big deal?

But, what happens to the chromosomal DNA in this process? Is it
denatureted by the dish detergent??

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