Re: how can i check a gene is either expressed or not or over expressed after applying the drugs

Since you're looking at a Histone Deacetylase Inhibitor, I guess you'd
really want to check its effect on the acetylation sites of the
target. This might be a useful reference:

Probing Lysine Acetylation in Proteins
Strategies, Limitations, and Pitfalls of in Vitro Acetyltransferase
Assays
http://mcponline.org/content/4/9/1226.full

On Dec 1, 8:13 pm, Nathan McCorkle <nmz...@gmail.com> wrote:
> if you can easily isolate the protein, maybe you can engineer an
> affinity tag onto the protein (though this could change natural
> transcription levels), you can probably detect binding via electron
> microscopy or Raman spectroscopy... if you can't do that (or don't
> want to), maybe you could try in vitro enzyme kinetics with an
> analogue chemical
>
> On Thu, Dec 1, 2011 at 2:24 AM, debadutta bhoi <debadutta1...@gmail.com> wrote:
> > i m actually doing project on a drug effect on cancer cells . the drug
> > is actually a Histone Deacetylase Inhibitor. so when i can i monitor
> > the drug is actually inhibiting the protein and  how can check the
> > some particular gene is expressed.
>
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> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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