Re: how can i check a gene is either expressed or not or over expressed after applying the drugs

if you can easily isolate the protein, maybe you can engineer an
affinity tag onto the protein (though this could change natural
transcription levels), you can probably detect binding via electron
microscopy or Raman spectroscopy... if you can't do that (or don't
want to), maybe you could try in vitro enzyme kinetics with an
analogue chemical

On Thu, Dec 1, 2011 at 2:24 AM, debadutta bhoi <debadutta1988@gmail.com> wrote:
> i m actually doing project on a drug effect on cancer cells . the drug
> is actually a Histone Deacetylase Inhibitor. so when i can i monitor
> the drug is actually inhibiting the protein and  how can check the
> some particular gene is expressed.
>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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