Whether you need a lid heater depends on the design of the machine.
Condensation will only occur when there is a temperature gradient.
This happens in PCR machines with a heat block, which cannot
homogenously heat the pcr tube.
On 6 feb, 09:53, Cathal Garvey <cathalgar...@gmail.com> wrote:
> OpenPCR has this design, also.
>
> I gather that without a heated lid or oil, sometimes in a long PCR you'd
> end up with practically the entire water content of the reaction in a
> droplet in the lid; the domed-cap lids in particular are perfectly
> shaped for holding a stable droplet!
>
> Mineral oil works surprisingly well though; I've had my kettle running
> at 55C for days now for G.stearothermophilus, and with some mineral oil
> on top there's been zero change in water level so far. :)
>
> On 05/02/12 23:54, Jeswin wrote:
>
>
>
>
>
>
>
>
>
> > I have noticed that on the Eppendorf at work, it allows for setting a
> > lid temperature and allows you to wait until that lid temp. is reached
> > before the program is run. Can someone tell me more about the effects
> > of the lid temperature? Is there a purpose, maybe more even heating?
>
> > On Sun, Feb 5, 2012 at 11:05 AM, Pieter <pietervanbohee...@gmail.com> wrote:
> >> Since we are developing a DIY rtPCR machine, I listed a few projects
> >> on our blog a while ago:http://www.amplino.org/2012/01/point-of-need-pcr-machine-round-up/.
>
> >> There are actually quite a lot diagnostics companies developing pcr
> >> machines for a kinds of purposes. Although this might not be regarded
> >> as DIY, still it indicates that PCR technology is still open to
> >> innovation.
>
> >> When building a PCR machine, just start out like Cathal indicated. A
> >> heater, a fan, temperature sensor and some processing unit is all you
> >> need to start out with to create your own thermocycler.
>
> >> The simpliest and quickest approach would be to use three water baths
> >> with thermostats, and just move the samples back en forth.
>
> >> On 4 feb, 19:52, Cathal Garvey <cathalgar...@gmail.com> wrote:
> >>> Cyclercan was dead easy to build, the code was a fun learning
> >>> experience, and it seems to perform beautifully on test-runs with the
> >>> CSV-output graphing function. However, I have not yet run a reaction in
> >>> it to confirm that the tubes reach the desired temperatures as quickly
> >>> as the temperature sensor does. So, I dunno.
>
> >>> The two biggest costs with Cyclercan were the Arduino and the Heatgun.
> >>> You could get a cheaper heatgun than the artist's one I used, but
> >>> probably not for less than 15, and Arduino clones could bring the costs
> >>> as low as 10 if you have your own FTDI-USB cable.
>
> >>> So I'd estimate the lowest you could go with a rig like mine (bearing in
> >>> mind that Ireland is affectionately known to its citizens as the "Ripoff
> >>> republic") would be:
> >>> 10 Arduino
> >>> 15 Heatgun
> >>> 5 PC Case-cooling fan
> >>> 2 for two solid-state relays
> >>> 10 12V supply for case-fan
> >>> 0.5 LM35 temperature sensor
> >>> = 42.5
>
> >>> I'm not counting the coffee can or any sealants used. Using a lightbulb
> >>> as done by Russell is definitely the way to go for price reduction. You
> >>> could also try mucking around with Nichrome in front of the cooler fan
> >>> for hot/cold circulation by the same fan, which would drop costs
> >>> significantly for a hot-air design.
>
> >>> Nichrome sufficient for this need would probably be a euro, but you'd
> >>> need to invest in a better power supply than the one figured above to
> >>> give the required current, which might only reduce the overall costs by
> >>> 5-10.
>
> >>> On 04/02/12 15:45, Jeswin wrote:
>
> >>>> So how many people have some type of DIYpcr machine?
>
> >>>> What kind and how does it perform so far? How hard was it to build?
> >>>> Any tips, problems encountered, and any ways to keep costs minimal
> >>>> would be nice to hear about.
>
> >>> --www.indiebiotech.com
> >>> twitter.com/onetruecathal
> >>> joindiaspora.com/u/cathalgarvey
> >>> PGP Public Key:http://bit.ly/CathalGKey
>
> >> --
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> --www.indiebiotech.com
> twitter.com/onetruecathal
> joindiaspora.com/u/cathalgarvey
> PGP Public Key:http://bit.ly/CathalGKey
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