OpenPCR has this design, also.
I gather that without a heated lid or oil, sometimes in a long PCR you'd
end up with practically the entire water content of the reaction in a
droplet in the lid; the domed-cap lids in particular are perfectly
shaped for holding a stable droplet!
Mineral oil works surprisingly well though; I've had my kettle running
at 55C for days now for G.stearothermophilus, and with some mineral oil
on top there's been zero change in water level so far. :)
On 05/02/12 23:54, Jeswin wrote:
> I have noticed that on the Eppendorf at work, it allows for setting a
> lid temperature and allows you to wait until that lid temp. is reached
> before the program is run. Can someone tell me more about the effects
> of the lid temperature? Is there a purpose, maybe more even heating?
>
> On Sun, Feb 5, 2012 at 11:05 AM, Pieter <pietervanboheemen@gmail.com> wrote:
>> Since we are developing a DIY rtPCR machine, I listed a few projects
>> on our blog a while ago: http://www.amplino.org/2012/01/point-of-need-pcr-machine-round-up/.
>>
>> There are actually quite a lot diagnostics companies developing pcr
>> machines for a kinds of purposes. Although this might not be regarded
>> as DIY, still it indicates that PCR technology is still open to
>> innovation.
>>
>> When building a PCR machine, just start out like Cathal indicated. A
>> heater, a fan, temperature sensor and some processing unit is all you
>> need to start out with to create your own thermocycler.
>>
>> The simpliest and quickest approach would be to use three water baths
>> with thermostats, and just move the samples back en forth.
>>
>> On 4 feb, 19:52, Cathal Garvey <cathalgar...@gmail.com> wrote:
>>> Cyclercan was dead easy to build, the code was a fun learning
>>> experience, and it seems to perform beautifully on test-runs with the
>>> CSV-output graphing function. However, I have not yet run a reaction in
>>> it to confirm that the tubes reach the desired temperatures as quickly
>>> as the temperature sensor does. So, I dunno.
>>>
>>> The two biggest costs with Cyclercan were the Arduino and the Heatgun.
>>> You could get a cheaper heatgun than the artist's one I used, but
>>> probably not for less than 15, and Arduino clones could bring the costs
>>> as low as 10 if you have your own FTDI-USB cable.
>>>
>>> So I'd estimate the lowest you could go with a rig like mine (bearing in
>>> mind that Ireland is affectionately known to its citizens as the "Ripoff
>>> republic") would be:
>>> 10 Arduino
>>> 15 Heatgun
>>> 5 PC Case-cooling fan
>>> 2 for two solid-state relays
>>> 10 12V supply for case-fan
>>> 0.5 LM35 temperature sensor
>>> = 42.5
>>>
>>> I'm not counting the coffee can or any sealants used. Using a lightbulb
>>> as done by Russell is definitely the way to go for price reduction. You
>>> could also try mucking around with Nichrome in front of the cooler fan
>>> for hot/cold circulation by the same fan, which would drop costs
>>> significantly for a hot-air design.
>>>
>>> Nichrome sufficient for this need would probably be a euro, but you'd
>>> need to invest in a better power supply than the one figured above to
>>> give the required current, which might only reduce the overall costs by
>>> 5-10.
>>>
>>> On 04/02/12 15:45, Jeswin wrote:
>>>
>>>> So how many people have some type of DIYpcr machine?
>>>
>>>> What kind and how does it perform so far? How hard was it to build?
>>>> Any tips, problems encountered, and any ways to keep costs minimal
>>>> would be nice to hear about.
>>>
>>> --www.indiebiotech.com
>>> twitter.com/onetruecathal
>>> joindiaspora.com/u/cathalgarvey
>>> PGP Public Key:http://bit.ly/CathalGKey
>>
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>
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