On Fri, Feb 3, 2012 at 1:22 PM, Jacob Shiach <kingjacob@gmail.com> wrote:
> The CaCl protocol you mentioned IS the protocol for making competent cells.
> So it will yield competent cells.
>
That's not totally true, CaCl2 is ONE method for making competent
E.coli cells, there are others, for instance those outlined in
"molecular cloning a laboratory manual" by Sambrook... competency
isn't an exact state either (as you said, CaCl2 transformation
protocol uses log-phase cells, but they're not really prepared for
maximum competency... some might not call them competent at all) .
Some methods prepare E.coli with special buffers and minimal medias
for CaCl2 TRANSFORMATION, some prepare cells for electroporation
transformation, or the PEG MgSO4 method that Cathal has told us about.
The third message from me in this post has all the info I compiled for
a class I taught where we used CaCl2 transformation:
http://groups.google.com/group/diybio/browse_thread/thread/fa9fd3c4df71d8d0?pli=1
--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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