On Wed, Feb 1, 2012 at 4:57 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> just use imageJ... there's gel lane analysis built-in, it gives you an
> intensity plot...
>
Cool, I gave it a try afterwards. It looks like I had 3:1
insert:vector rather than 2:1. Probably not a big deal.
I used the formula on this website to calculate how much insert DNA to
add. I then multiplied that value by 5 (the ratio of cut vector size
to insert size was 5:1).
http://www.methods.info/Methods/RNA_DNA/ligation_simple.html
The boss had a different method that I didn't really understand.
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