> When you order from the synth company, you get back your sequence
> insert only. We had the insert put into a plasmid, the whole plasmid
> sequenced, and we send back the plasmid DNA containing the insert they
> wanted.
Not meaning to knock your work, but that's what synth companies do, too.
You usually get your sequence back in some variant of pUC or blunt-ended
into pBluescript, and your sequence verified to ensure that it's correct.
--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey
--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment