On Thu, Feb 2, 2012 at 3:24 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> Behind the scenes they use phosphoramatide-(sp?)-based synthesis of
> 100-200bp oligonucleotides which are then assembled by PCR or ligation
> into the full sequence.
>
Boss did mention something along the lines of "They (synth companies)
use PCR too and when you do, you're bound towards some mistakes but
ours is cheaper"
> Beyond the use of high-quality inhouse oligosynthesis, their methods
> differ little from your methods might, except that:
> A) They specialise in just this job and problem (Maybe you do too?)
I guess we do, this is a PCR company and to fund the rest of the main
research we take on certain customer orders.
> B) Once you order, it's *their problem*! :)
When you order from the synth company, you get back your sequence
insert only. We had the insert put into a plasmid, the whole plasmid
sequenced, and we send back the plasmid DNA containing the insert they
wanted.
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