Re: [DIYbio] Re: DIY tube holder for vortex mixers

I don't see how a dremelfuge OR foam mounted on a common vortexer
could compete with this 20 kg monster homogenizer:
http://www.mpbio.com/product_info.php?family_key=116004500

now this thing is heavy which means it can shake the hell out of the
tubes without falling over, this means tissue will homogenize very
quickly

this doesn't mean a normal vortexer won't work, but how long will it
take? will heat/degradation of cell components start to be an issue
because its taking long?

On Thu, Feb 2, 2012 at 3:37 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> Despite my silence on the line, I am thinking of ways to remount a
> dremelfuge as/on a vortex.
>
> It's easy to shove an offset bearing into a dremelfuge; redesigning it
> for that wouldn't be a great problem in OpenSCAD. The problem arises
> when you spin it by whatever shaft goes through the bearing; you still
> need to hold the dremelfuge main body from rotating with the shaft, and
> the way you would do so would vary from drill/driver to drill/driver.
>
> If you have a pre-existing vortex model (where there's just a hub that
> oscillates without rotating) of course, it should be possible to
> engineer the Dremelfuge simply to fit on top without too much trouble.
> That's just a matter of replacing the dremel-adapted hub into a
> from-beneath mount matching the vortex's fitting.
>
> So, in brief; vortex, easy (drill + offset skate bearing + mount, I use
> a DIY'd one all the time). Unattended vortex, varies by design, haven't
> bothered making one yet. Dremelfuge-that-fits-to-existing-vortex, Easy,
> but I'd have to see pictures and precise measurements for the vortex mount.
>
> On 20/01/12 10:26, David McWilliam wrote:
>> Great post. I've been looking for something like this for macerating plant
>> material in bead beater tubes prior to DNA extraction. I've just  bought a
>> dremelfuge which arrived today and am thinking of ways to attach it to a
>> vortexer. The horizontal tube position would help maximise the bead
>> vortexing although it may need something to stop the tube caps from
>> popping. Maybe a vortexer modification of the dremelfuge - Cathal?
>> DMcW
>>
>>
>> On 20 January 2012 15:59, CodonAUG <elsbernd@gmail.com> wrote:
>>
>>> I wrote up a blog post about how to make a tube holder for vortex
>>> mixers.  I needed something to hold microcentrifuge tubes for me
>>> because I didn't want to stand there for 10 minutes with my arms
>>> getting uncomfortably shaken.
>>> Check it out if you are interested.
>>>
>>> http://cheapassscience.wordpress.com/2012/01/20/diy-tube-holder-for-vortex-mixers/
>>>
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>>
>
>
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>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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