[DIYbio] Fluorescent Microscope Incubator DIY

HI Everyone,

I have an issue in need of some collective help.
I am starting an experiment for grad thesis that will involve
protein(GFP, RFP) expression during specific cell cycles.
I would observe this and track expression with some blob/color
detection algorithms I'm assembling in OpenFrameworks. Assumption is
that this will all work and have access to a fluorescent microscope.
On to the question:
Timelapse will last about 60 hours, and the cells(murine fibroblasts)
need to be in incubation + CO2, so placing them on the stage for the
duration is not going to work.
I do have a proper incubator that meets those requirements but I dont
think putting a microscope in a humid warm incubator is good for the
objectives.
Outside of specialized microscopy incubator, has anyone tackled or
have any advice in maintaing incubation, condition and avoiding
condensation?
Looking for a hack/creative solution, or alternatively, around NYC, if
anyone is willing to provide access.
I can provide access to lasercutter in exchange :)

I found a pretty cool solution but may be a bit too technical for the
amount of time I have.
http://www.popsci.com/diy/article/2011-10/video-smart-petri-dish-images-cells-using-smartphone-camera-and-legos

Thanks so much,

akoz

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.