Forgive me if this has already been discussed, but what is the prevailing opinion on open-source enzymes. For doing DIY plasmid engineering, the most costly part is probably the restriction enzymes and ligase. Any kind of genetic manipulation (PCR, etc) gets expensive as a result of enzyme prices. Do you think it would be possible to develop some sort of open-source production method for useful enzymes (ie. open source vectors for expressing the enzymes and some easy way to purify them)? This is all just speculation, and my research into the subject has been limited. My experience in protein purification is also quite narrow.
I am aware that Cathal has an open-source plasmid vector in the works. If there is an effort to create open restriction enzymes, it would be convenient to coordinate them with the restriction sites on the plasmid. I am just looking for input on all aspects of such a project. What are the legal restrictions with gene patents? Do you think that it is viable to have some sort of chromatography set up that is reasonable for a large number of DIYers to have access too for purifying the proteins perhaps via a common tag? What do you think the costs (and benefits) of an endeavor like this might be? Thanks for your thoughts!
-Ethan
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