For eukaryotic cells I've used Trypan Blue, I think it should work for non-eukaryotes too:
http://en.wikipedia.org/wiki/Trypan_blue
Basically you'd take a mL of cell culture (may need to dilute if the cell concentrations are high), and add a drop of trypan blue (ideally a known amount), then place this under a hemacytometer and count the ratio of blue to non-blue cells.
You might also be able to use nigrosin or india ink in a negative stain manner.... maybe the burst/dead cells will appear shriveled?
http://en.wikipedia.org/wiki/Negative_stain
On Mar 8, 2012 8:02 AM, "medminus9" <harshsethia1989@gmail.com> wrote:
-- Is their any simple method to find out the ratio of live:dead bacteria
in your liquid culture?
Background:
My liquid culture contains lactobacillus bulgaricus.
Thanks!
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