[DIYbio] Re: Fluorescent Microscope Incubator DIY

Well the phone is there only as a source of light/ shifting. 
There is a sensor at bottom to scan the culture and that needs to be networked in.
Also the process involves burning off a layer from the ccd and afixing it in silicon and other such processes that I wouldn't have access to do reliably.


On Tuesday, March 6, 2012 9:39:51 AM UTC-5, naiverahim wrote:
it seems like the lego is there to secure the sample. why not just get a block of Styrofoam and cut a whole in it to place a small petri dish w/ cover in it. then just secure a smartphone over the petri dish, connect the phone to computer and do the same thing.

rahim

On Tuesday, March 6, 2012 2:16:54 AM UTC-5, Koz wrote:
HI Everyone,

I have an issue in need of some collective help.
I am starting an experiment for grad thesis that will involve
protein(GFP, RFP) expression during specific cell cycles.
I would observe this and track expression with some blob/color
detection algorithms I'm assembling in OpenFrameworks. Assumption is
that this will all work and have access to a fluorescent microscope.
On to the question:
Timelapse will last about 60 hours, and the cells(murine fibroblasts)
need to be in incubation + CO2, so placing them on the stage for the
duration is not going to work.
I do have a proper incubator that meets those requirements but I dont
think putting a microscope in a humid warm incubator is good for the
objectives.
Outside of specialized microscopy incubator, has anyone tackled or
have any advice in maintaing incubation, condition and avoiding
condensation?
Looking for a hack/creative solution, or alternatively, around NYC, if
anyone is willing to provide access.
I can provide access to lasercutter in exchange :)

I found a pretty cool solution but may be a bit too technical for the
amount of time I have.
http://www.popsci.com/diy/article/2011-10/video-smart-petri-dish-images-cells-using-smartphone-camera-and-legos

Thanks so much,

akoz

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