co2 controllers to mix the gas properly are many thousands of dollars.
you can just get tanks of premixed 5% CO2 air that works great for
incubation microscopy on the cheap. co2 independent media usually has
negative effects which can include cell death.
jordan
On Mar 6, 2012, at 12:14 PM, Koz <kozovski.a@gmail.com> wrote:
> That is a very interesting sketch and very similar to what I was
> thinking.
> My thought was to use the regulation from the main incubator and pipe
> the environment in.
> However I was worried that if there are any cold spots, it would
> result in condensation and a ruined image.
> I see the circular flow is meant to avoid this.
> Thanks, I'll start prototyping some things and see how it goes.
>
>
>
> On Mar 6, 10:39 am, John Griessen <j...@industromatic.com> wrote:
>> On 03/06/2012 01:16 AM, Koz wrote:
>>
>>> Timelapse will last about 60 hours, and the cells(murine fibroblasts)
>>> need to be in incubation + CO2, so placing them on the stage for the
>>> duration is not going to work.
>>
>> How about an incubator "slab" with support gear outside connected by tubes
>> to keep it warm/humid, and a second chamber around the main one to make
>> a double pane window to lessen chances of condensation?
>>
>> See sketch: http://ecosensory.com/diybio/stage_incubator-3.jpghttp://ecosensory.com/diybio/stage_incubator-3.gif
>
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