[DIYbio] Re: Positive control for GMO foods?

Hi. I daily perform gmo analyses on seeds.
I detect endogenous gene and p35S. Commonly I use an IRMM certified
positive control, such as a MON810 0.1% (it has a single copy of p35S)
The IRMM positive control is derived from gmo maize MON810 mixed with
negative maize. DNA must be extracted, obviously.
You can order it directly from
http://irmm.jrc.ec.europa.eu/reference_materials_catalogue/Pages/index.aspx
or in sigma aldrich.

Byebye

On 1 Feb, 18:17, Bugs <b...@binny.eu> wrote:
> Hi folks,
>
> I'm about to order some PCR primers that should let me detect the CaMV
> 35s promoter, a viral promoter that's very commonly used in making GM
> crops for food use. The idea, obviously, is a PCR-based test for foods
> containing GMOs. Er, or crops that just happen to have been infected
> by that virus, but I think it's limited to the cabbage family.
>
> I'm struggling to think of a positive control that I can use in my
> assay. I found a list of approved GM crops for the EU [1], but it's a
> bit opaque. Does anyone have any pointers toward GM crops orGMO-
> derived foods that I can buy in the UK?
>
> Many thanks,
> Bugs
>
> [1]http://ec.europa.eu/food/dyna/gm_register/index_en.cfm

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