As I repeated my pVIB-Transformation at university, you know, I used
far too less ampicillin.
Last week I plated the lawn on another lawn, containing more
ampicillin. Lawn again.
(I lost this week because my grandpa had gone)
so ASAP I'll put much more amp in LB Agar and repeat putting the
(partly transformed) bacterial lawn on it.
Maybe the ampicillin is too weak - or it doesn't work at all. I have
to find out....
What about the machine that Chris mentioned???
http://www.nature.com/srep/2012/120228/srep00289/full/srep00289.html#/t1
Electrospraying puts the plasmids into the cells, so that 70% of the
cells are transformed!!
If I can build a device that makes maybe 30% -50% efficiency, I don't
need selection markers any more?? I just have to make single
collonies on a petri dish, and the glowing ones will be the
transformants. With 30% efficiency, one out of three colonies should
be infected!!!
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