I recently read a summary review of 16s primers and their use in classification of new microbial species and they were talking about BLAST vs two other big databases. They stated that BLAST searching doesn't use a peer reviewed database, so anyone can submit sequences and label it whatever they want.
The point they made was, after producing a bunch of 16s gene fragments from their personal store of samples (known strains) and having them sequenced and then BLASTing them, I think BLAST showed errors more than 20% of the time. Whereas the other 2 databases that were peer checked agreed either 99% or 100% of the time with eachother.
Anyway, I know it doesn't answer your question, but maybe someone else knows of the other databases that are reviewed, might end up helping you in the long run perhaps because of better quality data you are checking against.
I can't find the article because it was in paper form somewhere around my room, if i find it i'll post it
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1 comments:
Better analysis has been made. Much impressive post.
Sample Analysis
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