On Fri, Mar 23, 2012 at 8:03 AM, Pat <eleten@gmail.com> wrote:
> Check it out when you get a chance: http://www.ncbi.nlm.nih.gov/guide/howto/design-pcr-primers/
>
Looking at the site, it seems that it checks the primer that you have
already built against the database. We need the opposite. We design
the primer based on the most common mutations for the WT sequence site
of interest which is known to us.
The primer should bind to the most commonly seen mutations.
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