Handy for ruling out nonspecific binding then but not for designing primers from scratch?
Jeswin <phillyj101@gmail.com> wrote:
>On Fri, Mar 23, 2012 at 8:03 AM, Pat <eleten@gmail.com> wrote:
>> Check it out when you get a chance:
>http://www.ncbi.nlm.nih.gov/guide/howto/design-pcr-primers/
>>
>Looking at the site, it seems that it checks the primer that you have
>already built against the database. We need the opposite. We design
>the primer based on the most common mutations for the WT sequence site
>of interest which is known to us.
>
>The primer should bind to the most commonly seen mutations.
>
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