It sounds like you're not so much interested in making methane as you are in trying to automate human fart production. True?
:)
Well it's certainly an unavoidable comparison. But the initial hope was to obliquely sonify the traces created by the microbes inside us (me) by linking it back to the methane whistle (similar acoustic effect as helium voices really), but I'm not sure sufficient hydrogen would be produced by gut microbes to leave an audible trace.
Instead of argon, I would use methane.
Also, and again forgive my ignorance, how is methane equivalent to methane for the purposes of maintaining an anaerobic environment
Thanks every for the helpful feedback so far
Best
Stephen
On Thursday, 22 March 2012 05:24:46 UTC, Simon Field wrote:
Instead of argon, I would use methane.It's a lot cheaper, and it is what you want in there anyway.-----Get a free science project every week! "http://scitoys.com/newsletter.html"
On Wed, Mar 21, 2012 at 7:44 PM, ByoWired wrote:
On Wednesday, March 21, 2012 4:46:39 PM UTC-4, Stephen Fortune wrote:
I need to use an anaerobic jar to grow a particularly difficult to culture strain of methanogen. They are completely anaerobic.
It seems to me you could de-gas your growth media by putting it under a vacuum for a while. Then purge with argon, tanks of which you can get at most welding places. After inoculating, you could purge the head space of your culture tubes (or whatever) with argon coming through a 0.2 micron filter, then perhaps, if your microbes can stand it, add a little bit of ascorbic acid to the media to scavenge any remaining oxygen. If I remember correctly, about 25 mg of ascorbic acid per 100 ml of water should do the trick.It sounds like you're not so much interested in making methane as you are in trying to automate human fart production. True?Be careful: just because it's a gas being made by microbes does not mean it's somehow safe because it's natural. Methane is methane no matter where it comes from, so the stuff can blow up in your face, burn down your house, or sear your eyeballs. Once it combines with air, it will be an explosive mixture.--To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/fF7woWnciH0J.
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