Re: [DIYbio] Re: DIY Anaerobe Jar? Is it possible?

On Fri, Mar 23, 2012 at 11:49 AM, Stephen Fortune
<stephen.j.fortune@gmail.com> wrote:
>> It sounds like you're not so much interested in making methane as you are
>> in trying to automate human fart production.  True?
>
>
> :)
>
> Well it's certainly an unavoidable comparison. But the initial hope was to
> obliquely sonify the traces created by the microbes inside us (me) by
> linking it back to the methane whistle (similar acoustic effect as helium
> voices really), but I'm not sure sufficient hydrogen would be produced by
> gut microbes to leave an audible trace.

You're thinking methane from your gut is coming up your throat?

>
> So in addition to having an air tight environment, is it advisable to purge
> the media which the microbes will be cultured in?
>

Won't hurt, if they don't need oxygen then get rid of it!

>
>> Instead of argon, I would use methane.
>
>
> Also, and again forgive my ignorance, how is methane equivalent to methane
> for the purposes of maintaining an anaerobic environment
>

I think you meant to ask how is argon equivalent to methane in
establishing an anaerobic environment... well, they're both
non-oxygen... argon is pretty much non-reactive, though as mentioned
Methane can explode.

LPG or propane would probably work similarly to methane, or maybe even
butane... hopefully these gasses don't melt plastic petri dishes
(you'd probably more than 1 ATM of pressure to do that with any
rapidity though)!

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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