Could you put filter over a scanner CCD, then just put the scanner in
the incubator with the plate on top... a scan would be not be a single
moment snapshot, but its probably think enough to fit in the incubator
On Tue, Mar 6, 2012 at 2:16 AM, Koz <kozovski.a@gmail.com> wrote:
> HI Everyone,
>
> I have an issue in need of some collective help.
> I am starting an experiment for grad thesis that will involve
> protein(GFP, RFP) expression during specific cell cycles.
> I would observe this and track expression with some blob/color
> detection algorithms I'm assembling in OpenFrameworks. Assumption is
> that this will all work and have access to a fluorescent microscope.
> On to the question:
> Timelapse will last about 60 hours, and the cells(murine fibroblasts)
> need to be in incubation + CO2, so placing them on the stage for the
> duration is not going to work.
> I do have a proper incubator that meets those requirements but I dont
> think putting a microscope in a humid warm incubator is good for the
> objectives.
> Outside of specialized microscopy incubator, has anyone tackled or
> have any advice in maintaing incubation, condition and avoiding
> condensation?
> Looking for a hack/creative solution, or alternatively, around NYC, if
> anyone is willing to provide access.
> I can provide access to lasercutter in exchange :)
>
> I found a pretty cool solution but may be a bit too technical for the
> amount of time I have.
> http://www.popsci.com/diy/article/2011-10/video-smart-petri-dish-images-cells-using-smartphone-camera-and-legos
>
> Thanks so much,
>
> akoz
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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