What do you plan on using for the silica columns? I don't think the
companies (e.g. QIAGEN) don't sell columns outside of kits. Or are you
using a generic silica column, under the assumption that MiniPrep
columns are the same.
On Mon, Mar 26, 2012 at 2:48 PM, Mac Cowell <mac@diybio.org> wrote:
> Yeah that sounds great. Guanidine hydrochloride is considered toxic waste by
> most university labs, so a drain safe alternative is great.
>
> Mac
>
> 231.313.9062 // @100ideas // sent from my rotary phone
>
> On Mar 25, 2012, at 10:22 PM, Derek <derekja@gmail.com> wrote:
>
> Wow, Cory, that's great! I look forward to reading and trying that.
>
> On Sunday, 25 March 2012 20:46:10 UTC-7, cory....@gmail.com wrote:
>>
>> No autoclaving necessary.
>>
>> Also, I wrote an article for the next volume of Citizen Science
>> Quarterly, which should be out shortly I think, that has recipes for
>> all the miniprep solutions using only household ingredients. I found
>> that sodium chloride works just fine in place of guanidine
>> hydrochloride as long as the concentration of salt in the final
>> mixture (buffer P1 + P2 + N3) is above 2M and the pH is below 5.5. My
>> homemade recipe for N3 is 11.5g table salt, 3.7g potassium chloride,
>> and 43mL of distilled white vinegar (no extra water added). You can
>> get the potassium chloride at the grocery store (I found it at
>> Ralphs). It's sold by Morton Salt as some sort of additive for water
>> softener tanks. Anyways, if you give it a try let me know how it
>> works.
>>
>> Also, an added benefit of using the NaCl is that very little RNA is
>> bound to the silica compared to guanidine (which is why kits come with
>> RNase to add to buffer P1). There is a little RNA that comes along
>> for the ride, but I imagine if you play around the the pH and salt
>> concentration you can probably minimize that even more.
>>
>> -cory
>
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