> What do you plan on using for the silica columns? I don't think the
> companies (e.g. QIAGEN) don't sell columns outside of kits. Or are you
> using a generic silica column, under the assumption that MiniPrep
> columns are the same.
You can use diatomaceous earth (sold at pool supply stores). Instead
of packing them into a column and centrifuging the liquid through the
column you add the diatomaceous earth powder to the solution, vortex
to suspend it, then quickly centrifuge to pellet the diatomaceous
earth, then either pour or pipette away the supernatant. As described
here:
Boom, R., Sol, C. J., Salimans, M. M., Jansen, C. L., Wertheim-van
Dillen, P. M., & van Der Noordaa, J. (1990). Rapid and simple method
for purification of nucleic acids. Journal of Clinical Microbiology,
28(3), 495-503. Retrieved from
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=269651&tool=pmcentrez&rendertype=abstract
-cory
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