Does your kit require a centrifuge for the CaCl2 rinses? Or have you
got around that with a tweaked protocol?
On Thu, Mar 8, 2012 at 7:20 PM, Matías Gutiérrez
<matiasgutierrezm@gmail.com> wrote:
> Also included, forgot to add it into the list. :) but not for propagation,
> the propagation is achieved by plating the bacteria in the solid LB media.
> The liquid LB is used for recovery after heat shock transformation.
>
> Basically the idea is that you'll have everything you need, we've tested
> these in classrooms for over 3 years with great results and have improved
> our kit each year, this would be the first version that is not meant for the
> classroom but for enthusiasts!
>
> We could also provide individual components in a convenient ammount so that
> ppl don't need to buy bulk like research labs usually do.
>
> Matías
>
>
> On Thursday, March 8, 2012 8:55:43 PM UTC-3, Nathan McCorkle wrote:
>>
>> Matias, what about liquid LB for propagation?
>>
>> On Thu, Mar 8, 2012 at 6:46 PM, Matías Gutiérrez
>> <matiasgutierrezm@gmail.com> wrote:
>> > Hey Guys,
>> >
>> > First of all thanks to Edwin for your warm wishes :)
>> >
>> > Now regarding these kits, they basically include everything.
>> >
>> > For example the GFP cloning kit includes the following:
>> >
>> > plasmid encoding GFP and ampicilling resistance (GFP expression is
>> > induced
>> > by arabinose)
>> > bacterial stock culture (stab) that needs to be propagated before the
>> > experiment
>> > 2 plates containing propagation media (LB)
>> > plate containing selection media (LB + ampicillin)
>> > plate containing selection media and inducer (LB + ampicilling +
>> > arabinose)
>> > inoculations loops, microtubes, transfer pipettes, etc
>> > detailed protocol for the experiment
>> > Slide show with explanation of the protocol
>> > further reading material
>> >
>> > If we can work out a good distribuition network I'd really like to make
>> > this
>> > available for the community at a low cost (hopefully less than
>> > USD$30.00)
>> >
>> > Matías
>> >
>> > On Thursday, March 8, 2012 6:29:05 PM UTC-3, Avery wrote:
>> >>
>> >> You might just want to get pGREEN from carolina. There is no need for
>> >> arabinose with that plasmid.
>> >>
>> >> --Avery
>> >>
>> >> On Thu, Mar 8, 2012 at 3:48 PM, Mega <masterstorm123@gmail.com> wrote:
>> >>>
>> >>> So you are still dependent on chemical companies that may deny
>> >>> access.
>> >>>
>> >>> And if you (vacuum) destill the apple juice so the concentration gets
>> >>> higher? Is arab heat stable (say 100°C)?
>> >>>
>> >>> On 8 Mrz., 20:20, Derek <dere...@gmail.com> wrote:
>> >>> > Nope, no arabinose at the supermarket. It's found in low
>> >>> > concentrations in grape and apple juice, but I wasn't able to get
>> >>> > sufficient concentrations to enable GFP production.
>> >>> >
>> >>> > Best price I found, and nice people to deal with, are the folks at
>> >>> > Cascade Biochemicals,http://www.cascadebiochems.com/
>> >>> >
>> >>> > --Derek
>> >>> >
>> >>> > On Mar 8, 9:26 am, Mega <masterstorm...@gmail.com> wrote:
>> >>> >
>> >>> >
>> >>> >
>> >>> >
>> >>> >
>> >>> >
>> >>> >
>> >>> > > As for 2. GFP I want to mention, you need arabinose.
>> >>> >
>> >>> > > You can get it as DIY biologist, but not from the supermarket ( I
>> >>> > > think so, in fact never looked for arab there)
>> >>> >
>> >>> > > And you need a black light source.
>> >>> >
>> >>> > > There's a plasmid, pVIB, that is always 'on' and needs no special
>> >>> > > substrate. It may be more attracting to people if they see it
>> >>> > > glowing
>> >>> > > in the dark without UV-Lamps.
>> >>> > > That may only be true for me, at all. Though, I recommend thinking
>> >>> > > about this.
>> >>> >
>> >>> > > Best
>> >>>
>> >>> --
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>> >>
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>> --
>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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