On Thu, Mar 8, 2012 at 3:12 PM, Mega <masterstorm123@gmail.com> wrote:
> Is this really sterile/aseptic?
>
> Because why would university use the autoclavator if it's that easy?
>
> Have you never had significant contamination using this technique??
>
No problems with E coli transformations. I did this way and had my
plasmids sequenced, and they came out correct. No contamination. Have
done this way many times.
I am not sure but maybe autoclaves are for mammalian cell cultures?
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