Re: [DIYbio] Re: Experiment with DNA and learn the techniques and concepts in a convenient and easy way

Matias, what about liquid LB for propagation?

On Thu, Mar 8, 2012 at 6:46 PM, Matías Gutiérrez
<matiasgutierrezm@gmail.com> wrote:
> Hey Guys,
>
>    First of all thanks to Edwin for your warm wishes :)
>
> Now regarding these kits, they basically include everything.
>
> For example the GFP cloning kit includes the following:
>
> plasmid encoding GFP and ampicilling resistance (GFP expression is induced
> by arabinose)
> bacterial stock culture (stab) that needs to be propagated before the
> experiment
> 2 plates containing propagation media (LB)
> plate containing selection media (LB + ampicillin)
> plate containing selection media and inducer (LB + ampicilling + arabinose)
> inoculations loops, microtubes, transfer pipettes, etc
> detailed protocol for the experiment
> Slide show with explanation of the protocol
> further reading material
>
> If we can work out a good distribuition network I'd really like to make this
> available for the community at a low cost (hopefully less than USD$30.00)
>
> Matías
>
> On Thursday, March 8, 2012 6:29:05 PM UTC-3, Avery wrote:
>>
>> You might just want to get pGREEN from carolina.  There is no need for
>> arabinose with that plasmid.
>>
>> --Avery
>>
>> On Thu, Mar 8, 2012 at 3:48 PM, Mega <masterstorm123@gmail.com> wrote:
>>>
>>> So you are still dependent on chemical companies   that may deny
>>> access.
>>>
>>> And if you (vacuum) destill the apple juice so the concentration gets
>>> higher? Is arab heat stable (say 100°C)?
>>>
>>> On 8 Mrz., 20:20, Derek <dere...@gmail.com> wrote:
>>> > Nope, no arabinose at the supermarket. It's found in low
>>> > concentrations in grape and apple juice, but I wasn't able to get
>>> > sufficient concentrations to enable GFP production.
>>> >
>>> > Best price I found, and nice people to deal with, are the folks at
>>> > Cascade Biochemicals,http://www.cascadebiochems.com/
>>> >
>>> > --Derek
>>> >
>>> > On Mar 8, 9:26 am, Mega <masterstorm...@gmail.com> wrote:
>>> >
>>> >
>>> >
>>> >
>>> >
>>> >
>>> >
>>> > > As for 2. GFP I want to mention, you need arabinose.
>>> >
>>> > > You can get it as DIY biologist, but not from the supermarket ( I
>>> > > think so, in fact never looked for arab there)
>>> >
>>> > > And you need a black light source.
>>> >
>>> > > There's a plasmid, pVIB, that is always 'on' and needs no special
>>> > > substrate. It may be more attracting to people if they see it glowing
>>> > > in the dark without UV-Lamps.
>>> > > That may only be true for me, at all. Though, I recommend thinking
>>> > > about this.
>>> >
>>> > > Best
>>>
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>>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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