I think the kits sound amazing! One I would be interested in for my
own purposes is a genetic analysis kit that would allow comparison of
human dna for various traits. I would be happy to discuss in more
detail off-list if you like.
Heather
On Mar 8, 6:46 pm, Matías Gutiérrez <matiasgutierr...@gmail.com>
wrote:
> Hey Guys,
>
> First of all thanks to Edwin for your warm wishes :)
>
> Now regarding these kits, they basically include everything.
>
> For example the GFP cloning kit includes the following:
>
> - plasmid encoding GFP and ampicilling resistance (GFP expression is
> induced by arabinose)
> - bacterial stock culture (stab) that needs to be propagated before the
> experiment
> - 2 plates containing propagation media (LB)
> - plate containing selection media (LB + ampicillin)
> - plate containing selection media and inducer (LB + ampicilling +
> arabinose)
> - inoculations loops, microtubes, transfer pipettes, etc
> - detailed protocol for the experiment
> - Slide show with explanation of the protocol
> - further reading material
>
> If we can work out a good distribuition network I'd really like to make
> this available for the community at a low cost (hopefully less than
> USD$30.00)
>
> Matías
>
>
>
>
>
>
>
> On Thursday, March 8, 2012 6:29:05 PM UTC-3, Avery wrote:
>
> > You might just want to get pGREEN from carolina. There is no need for
> > arabinose with that plasmid.
>
> > --Avery
>
> > On Thu, Mar 8, 2012 at 3:48 PM, Mega <masterstorm...@gmail.com> wrote:
>
> >> So you are still dependent on chemical companies that may deny
> >> access.
>
> >> And if you (vacuum) destill the apple juice so the concentration gets
> >> higher? Is arab heat stable (say 100°C)?
>
> >> On 8 Mrz., 20:20, Derek <dere...@gmail.com> wrote:
> >> > Nope, no arabinose at the supermarket. It's found in low
> >> > concentrations in grape and apple juice, but I wasn't able to get
> >> > sufficient concentrations to enable GFP production.
>
> >> > Best price I found, and nice people to deal with, are the folks at
> >> > Cascade Biochemicals,http://www.cascadebiochems.com/
>
> >> > --Derek
>
> >> > On Mar 8, 9:26 am, Mega <masterstorm...@gmail.com> wrote:
>
> >> > > As for 2. GFP I want to mention, you need arabinose.
>
> >> > > You can get it as DIY biologist, but not from the supermarket ( I
> >> > > think so, in fact never looked for arab there)
>
> >> > > And you need a black light source.
>
> >> > > There's a plasmid, pVIB, that is always 'on' and needs no special
> >> > > substrate. It may be more attracting to people if they see it glowing
> >> > > in the dark without UV-Lamps.
> >> > > That may only be true for me, at all. Though, I recommend thinking
> >> > > about this.
>
> >> > > Best
>
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