Hey Guys,
First of all thanks to Edwin for your warm wishes :)
Now regarding these kits, they basically include everything.
For example the GFP cloning kit includes the following:
- plasmid encoding GFP and ampicilling resistance (GFP expression is induced by arabinose)
- bacterial stock culture (stab) that needs to be propagated before the experiment
- 2 plates containing propagation media (LB)
- plate containing selection media (LB + ampicillin)
- plate containing selection media and inducer (LB + ampicilling + arabinose)
- inoculations loops, microtubes, transfer pipettes, etc
- detailed protocol for the experiment
- Slide show with explanation of the protocol
- further reading material
If we can work out a good distribuition network I'd really like to make this available for the community at a low cost (hopefully less than USD$30.00)
Matías
On Thursday, March 8, 2012 6:29:05 PM UTC-3, Avery wrote:
You might just want to get pGREEN from carolina. There is no need for arabinose with that plasmid.
--AveryOn Thu, Mar 8, 2012 at 3:48 PM, Mega <masterstorm123@gmail.com> wrote:
So you are still dependent on chemical companies that may deny
access.
And if you (vacuum) destill the apple juice so the concentration gets
higher? Is arab heat stable (say 100°C)?
> Cascade Biochemicals,http://www.
On 8 Mrz., 20:20, Derek <dere...@gmail.com> wrote:
> Nope, no arabinose at the supermarket. It's found in low
> concentrations in grape and apple juice, but I wasn't able to get
> sufficient concentrations to enable GFP production.
>
> Best price I found, and nice people to deal with, are the folks at
cascadebiochems.com/
>
> --Derek
>
> On Mar 8, 9:26 am, Mega <masterstorm...@gmail.com> wrote:
>
>
>
>
>
>
>
> > As for 2. GFP I want to mention, you need arabinose.
>
> > You can get it as DIY biologist, but not from the supermarket ( I
> > think so, in fact never looked for arab there)
>
> > And you need a black light source.
>
> > There's a plasmid, pVIB, that is always 'on' and needs no special
> > substrate. It may be more attracting to people if they see it glowing
> > in the dark without UV-Lamps.
> > That may only be true for me, at all. Though, I recommend thinking
> > about this.
>
> > Best
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