Re: [DIYbio] Re: Experiment with DNA and learn the techniques and concepts in a convenient and easy way

You might just want to get pGREEN from carolina.  There is no need for arabinose with that plasmid.

--Avery

On Thu, Mar 8, 2012 at 3:48 PM, Mega <masterstorm123@gmail.com> wrote:
So you are still dependent on chemical companies   that may deny
access.

And if you (vacuum) destill the apple juice so the concentration gets
higher? Is arab heat stable (say 100°C)?

On 8 Mrz., 20:20, Derek <dere...@gmail.com> wrote:
> Nope, no arabinose at the supermarket. It's found in low
> concentrations in grape and apple juice, but I wasn't able to get
> sufficient concentrations to enable GFP production.
>
> Best price I found, and nice people to deal with, are the folks at
> Cascade Biochemicals,http://www.cascadebiochems.com/
>
> --Derek
>
> On Mar 8, 9:26 am, Mega <masterstorm...@gmail.com> wrote:
>
>
>
>
>
>
>
> > As for 2. GFP I want to mention, you need arabinose.
>
> > You can get it as DIY biologist, but not from the supermarket ( I
> > think so, in fact never looked for arab there)
>
> > And you need a black light source.
>
> > There's a plasmid, pVIB, that is always 'on' and needs no special
> > substrate. It may be more attracting to people if they see it glowing
> > in the dark without UV-Lamps.
> > That may only be true for me, at all. Though, I recommend thinking
> > about this.
>
> > Best

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.


--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment