Re: [DIYbio] Re: Fluorescent Microscope Incubator DIY

The humidity may be easily finessed if the cells grow well at 100% humidity.


Warm the air/COto the desired temperature, bubble it through

a warm water bath, and send it into the incubator cell. The temperature
drop between the source and the incubator will keep the humidity at
100%.

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On Tue, Mar 6, 2012 at 10:57 AM, John Griessen <john@industromatic.com> wrote:
On 03/06/2012 11:14 AM, Koz wrote:
I see the circular flow is meant to avoid this.
 Sure.  Just flow enough to get turbulent stirring inside and
even temps.  Try to find out as much about the dimensions of the
Warner instruments stage so you can benefit from their
past developments.  Search for what they have patented and how
long ago.  Avoid using their current patented concepts.


On 03/06/2012 11:23 AM, Jordan Miller wrote:
> co2 controllers to mix the gas properly are many thousands of dollars.

But, you could make a CO2-stat fairly quickly and use a one time measurement
with a CO2 meter lab instrument to calibrate it every so often.  The open loop
flow of CO2 is going to have problems of how do you get the humidity
just right as well as the CO2 balance, so it's not a cure all.


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