The top window should be glass, and should be 0.17 mm thick or less.
Most microscope objectives are designed to work with glass coverslips
of this thickness. See
An acrylic window 1/8 inch thick won't allow the objective to get close enough
to even focus on the cells beneath at any decent magnification.
The bottom window should be a normal microscope slide, especially if an oil
immersion condenser is used.
The trick of using double-stick tape is still quite useful -- but you don't need
a laser cutter. Just cut the tape to the shape you want, stick it onto a slide,
and stick a cover slip on top. The gas escape holes are simply cut into the
tape, instead of into the top or bottom window. Scissors or an Xacto knife
will do for cutting the tape.
-----
Get a free science project every week! "http://scitoys.com/newsletter.html"On Tue, Mar 6, 2012 at 10:53 AM, Chris Templeman <christempleman@gmail.com> wrote:
Since you mentioned you had a laser cutter check out the openspectrometer.com guys and in particular the third video on their site. They show a pretty clever way to make 'cuvettes' or simple holders for the spectrometer, but the design would lend itself well to being scaled up a little bit to make a cell culture holder that you would mate to a microscope since it has two 'optical surfaces'. Further the C02 could be pipe in via larger holes then what they show in the movie.ChrisTo view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/uuhAWOe7X9MJ.--
On Tuesday, March 6, 2012 12:14:24 PM UTC-5, Koz wrote:That is a very interesting sketch and very similar to what I was
thinking.
My thought was to use the regulation from the main incubator and pipe
the environment in.
However I was worried that if there are any cold spots, it would
result in condensation and a ruined image.
I see the circular flow is meant to avoid this.
Thanks, I'll start prototyping some things and see how it goes.
On Mar 6, 10:39 am, John Griessen <j...@industromatic.com> wrote:
> On 03/06/2012 01:16 AM, Koz wrote:
>
> > Timelapse will last about 60 hours, and the cells(murine fibroblasts)
> > need to be in incubation + CO2, so placing them on the stage for the
> > duration is not going to work.
>
> How about an incubator "slab" with support gear outside connected by tubes
> to keep it warm/humid, and a second chamber around the main one to make
> a double pane window to lessen chances of condensation?
>
> See sketch: http://ecosensory.com/diybio/stage_incubator-3.jpghttp://ecosensory.com/diybio/stage_incubator-3.gif
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