Mega,
you should just try to add E.coli from a plate to sterile water in a
microtube. Add your DNA, then place the electrodes from a piezo
sparker in and spark 1-3 times (or have 3 tubes, and try 1 spark on
tube #1, 2 sparks on tube #2, 3 sparks on tube #3). Then add some LB,
and incubate for 1 hr... then plate that on LB + amp plates.
No CaCl2 or anything... its an experiment, but you could be the first
to actually try it! (we've talked about it a lot on here, but never
tried it)
On Sat, Mar 10, 2012 at 8:19 AM, Mega <masterstorm123@gmail.com> wrote:
> @
> Chris:
>
> That's amazing!!!
>
> Did I understand that correctly? Usually you transform roughly 1
> cell and 999 stay untransformed. Using this technique, ~600 out of
> 1000 cells are transformed???
>
> If that is true, I must ask a friend of mine (electrical engineer)
> if he is interested to build such a miracle device!!!!!
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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