On Sat, Mar 10, 2012 at 2:32 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
>>
>> What do you mean by rinsing with water?
>
> From my LB liquid culture I centrifuged, decanted supernatant,
> resuspended in distilled/sterile/nanopure water, repeat once or twice
>
So these cells are the regular, hardy kind? The competent cells
normally used are treated with utmost care, like no rapid temperature
changes, and they lose transformation efficiency per freeze/thaw
cycle.
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