On Sat, Mar 10, 2012 at 1:05 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> Jm109 or DH5-alpha or K12... whatever you have on hand.... i did this
> once (with a commercial electroporator) and it worked wayyyy better
> than CaCl2
>
Where do you get those from?
The ones I use are New England Biolabs Competent E coli. Are they
special strains that have improved transformation efficiency over the
DH5-alpha, etc?
> just make sure its in exponential phase growth, that's all
>
> I rinsed my e. coli 2 or 3 times with pure water before adding DNA and
> electroporating, but Mega says he doesn't have a centrifuge... and if
What do you mean by rinsing with water?
--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment