Re: [DIYbio] Re: Making my own incubator inexpensively

I'm not arguing with Cathal -- he's brilliant, and more knowledgeable than I am

about these things. But I can build an incubator that guarantees I will never heat

shock my critters, without needing better than 2 or 3 degrees in accuracy. I just

set the temp 4 degrees below the heat shock temperature, and measure the growth

of the colony more directly, instead of using temperature as a proxy for growth.

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On Tue, Apr 24, 2012 at 9:45 PM, Nathan McCorkle <nmz787@gmail.com> wrote:

On Tue, Apr 24, 2012 at 12:54 PM, Simon Quellen Field
<sfield@scitoys.com> wrote:
> What you say is true, but it is not necessary.
>

> You are using temperature as a surrogate for something else you actually
> should be
> measuring instead, such as the number of organisms per unit volume, or the
> growth
> rate, or the amount of some metabolite exceeds a threshold.

> On Tue, Apr 24, 2012 at 1:20 AM, Cathal Garvey <cathalgarvey@gmail.com>
> wrote:

>> Higher temperatures mean more
>> reactions per second, meaning faster growth. However, any higher than
>> this ceiling, and heat-shock starts to get induced. While the cells will
>> survive, their gene expression profiles will change markedly.

What Cathal is saying here is a combination of things, that the temp
effects the growth rate so you could look at colony size delta with
vision processing or cell density with a light meter, but gene
expression IS heat dependent... when free energy levels are over a
protein or ribozyme's threshold (whatever that may be), stuff can
happen in a marked way (i.e. HSPs)

--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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