Haven't tried anything out yet but as soon as the first set of plates arrives I will give it a go and report back. I'm not sure that including water is necessary though. If the only water in the oven is in the microbes they will get a disporportionate amount of the microwave energy. I'm assuming 2min would be enought to cook any residual surface bacteria to a crisp.
Zeb
Zeb
> From: nmz787@gmail.com
> Date: Tue, 24 Apr 2012 21:29:03 -0400
> Subject: Re: [DIYbio] Recycling micro plates
> To: diybio@googlegroups.com
>
> Actually that sounds pretty good, did you microwave them with water
> inside too. How long?
>
> My guess would be on low to med settings for 10-15 minutes (enough so
> they get hot, without melting the plastic).
>
>
> On Tue, Apr 24, 2012 at 6:52 PM, Zebedee Boy <zebedeeboy@hotmail.com> wrote:
> > I was thinking wash, clean and dry before microwaving (as per the diybio FAQ
> > - I read it first!
> > http://openwetware.org/wiki/DIYbio/FAQ/Methods#Microwave_sterilization_of_plastic_tissue_culture_vessels_for_reuse).
> > I've microwaved clean tissue culture plastic to sterilise it before but not
> > used micro plates. So just wanted to see if anyone else had tried before I
> > give it a go.
> >
> > Zeb
> >
> >> Subject: Re: [DIYbio] Recycling micro plates
> >> From: cathalgarvey@gmail.com
> >> Date: Tue, 24 Apr 2012 17:26:41 +0100
> >> To: diybio@googlegroups.com
> >
> >>
> >> Go invest in some nice glass dishes. Expensive up front, but infinitely
> >> reusable. :)
> >>
> >> Robert O'Callahan <ropoctl@rice.edu> wrote:
> >>
> >> >Polystyrene will not survive autoclaving. They use ethylene oxide,
> >> >but that might be a bit out of reach for hobbyists.
> >> >
> >> >-Rob
> >> >
> >> >On Tue, Apr 24, 2012 at 10:37 AM, Zebedee Boy <zebedeeboy@hotmail.com>
> >> >wrote:
> >> >> Has anyone come up with a way to recycle the plastic plates used for
> >> >agar
> >> >> plates? Can I just flip the used agar out and heat sterilise before
> >> >using
> >> >> again?
> >> >>
> >> >> Zeb
> >> >>
> >> >> --
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> >> >
> >> >
> >> >
> >> >--
> >> >Rob O'Callahan
> >> >M.S. Civil & Environmental Engineering 2012
> >> >George R. Brown School of Engineering | Rice University
> >> >E: rpo1@rice.edu<mailto:rpo1@rice.edu>| P: 847.641.1285
> >> >
> >> >--
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> >>
> >> --
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>
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics
>
> --
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
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>
> Date: Tue, 24 Apr 2012 21:29:03 -0400
> Subject: Re: [DIYbio] Recycling micro plates
> To: diybio@googlegroups.com
>
> Actually that sounds pretty good, did you microwave them with water
> inside too. How long?
>
> My guess would be on low to med settings for 10-15 minutes (enough so
> they get hot, without melting the plastic).
>
>
> On Tue, Apr 24, 2012 at 6:52 PM, Zebedee Boy <zebedeeboy@hotmail.com> wrote:
> > I was thinking wash, clean and dry before microwaving (as per the diybio FAQ
> > - I read it first!
> > http://openwetware.org/wiki/DIYbio/FAQ/Methods#Microwave_sterilization_of_plastic_tissue_culture_vessels_for_reuse).
> > I've microwaved clean tissue culture plastic to sterilise it before but not
> > used micro plates. So just wanted to see if anyone else had tried before I
> > give it a go.
> >
> > Zeb
> >
> >> Subject: Re: [DIYbio] Recycling micro plates
> >> From: cathalgarvey@gmail.com
> >> Date: Tue, 24 Apr 2012 17:26:41 +0100
> >> To: diybio@googlegroups.com
> >
> >>
> >> Go invest in some nice glass dishes. Expensive up front, but infinitely
> >> reusable. :)
> >>
> >> Robert O'Callahan <ropoctl@rice.edu> wrote:
> >>
> >> >Polystyrene will not survive autoclaving. They use ethylene oxide,
> >> >but that might be a bit out of reach for hobbyists.
> >> >
> >> >-Rob
> >> >
> >> >On Tue, Apr 24, 2012 at 10:37 AM, Zebedee Boy <zebedeeboy@hotmail.com>
> >> >wrote:
> >> >> Has anyone come up with a way to recycle the plastic plates used for
> >> >agar
> >> >> plates? Can I just flip the used agar out and heat sterilise before
> >> >using
> >> >> again?
> >> >>
> >> >> Zeb
> >> >>
> >> >> --
> >> >> You received this message because you are subscribed to the Google
> >> >Groups
> >> >> "DIYbio" group.
> >> >> To post to this group, send email to diybio@googlegroups.com.
> >> >> To unsubscribe from this group, send email to
> >> >> diybio+unsubscribe@googlegroups.com.
> >> >> For more options, visit this group at
> >> >> http://groups.google.com/group/diybio?hl=en.
> >> >
> >> >
> >> >
> >> >--
> >> >Rob O'Callahan
> >> >M.S. Civil & Environmental Engineering 2012
> >> >George R. Brown School of Engineering | Rice University
> >> >E: rpo1@rice.edu<mailto:rpo1@rice.edu>| P: 847.641.1285
> >> >
> >> >--
> >> >You received this message because you are subscribed to the Google
> >> >Groups "DIYbio" group.
> >> >To post to this group, send email to diybio@googlegroups.com.
> >> >To unsubscribe from this group, send email to
> >> >diybio+unsubscribe@googlegroups.com.
> >> >For more options, visit this group at
> >> >http://groups.google.com/group/diybio?hl=en.
> >>
> >> --
> >> Sent from K-9 Mail on Android
> >>
> >> --
> >> You received this message because you are subscribed to the Google Groups
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> >>
> >
> > --
> > You received this message because you are subscribed to the Google Groups
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>
>
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics
>
> --
> You received this message because you are subscribed to the Google Groups "DIYbio" group.
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>
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