RE: [DIYbio] Recycling micro plates

Spores often have a low our negligible water content, and "wet heat" in the form of superheated steam is a great way to kill them. You are probably right about other cells though.

Zebedee Boy <zebedeeboy@hotmail.com> wrote:

>
>Haven't tried anything out yet but as soon as the first set of plates
>arrives I will give it a go and report back. I'm not sure that
>including water is necessary though. If the only water in the oven is
>in the microbes they will get a disporportionate amount of the
>microwave energy. I'm assuming 2min would be enought to cook any
>residual surface bacteria to a crisp. Zeb
> > From: nmz787@gmail.com
>> Date: Tue, 24 Apr 2012 21:29:03 -0400
>> Subject: Re: [DIYbio] Recycling micro plates
>> To: diybio@googlegroups.com
>>
>> Actually that sounds pretty good, did you microwave them with water
>> inside too. How long?
>>
>> My guess would be on low to med settings for 10-15 minutes (enough so
>> they get hot, without melting the plastic).
>>
>>
>> On Tue, Apr 24, 2012 at 6:52 PM, Zebedee Boy <zebedeeboy@hotmail.com>
>wrote:
>> > I was thinking wash, clean and dry before microwaving (as per the
>diybio FAQ
>> > - I read it first!
>> >
>http://openwetware.org/wiki/DIYbio/FAQ/Methods#Microwave_sterilization_of_plastic_tissue_culture_vessels_for_reuse).
>> > I've microwaved clean tissue culture plastic to sterilise it before
>but not
>> > used micro plates. So just wanted to see if anyone else had tried
>before I
>> > give it a go.
>> >
>> > Zeb
>> >
>> >> Subject: Re: [DIYbio] Recycling micro plates
>> >> From: cathalgarvey@gmail.com
>> >> Date: Tue, 24 Apr 2012 17:26:41 +0100
>> >> To: diybio@googlegroups.com
>> >
>> >>
>> >> Go invest in some nice glass dishes. Expensive up front, but
>infinitely
>> >> reusable. :)
>> >>
>> >> Robert O'Callahan <ropoctl@rice.edu> wrote:
>> >>
>> >> >Polystyrene will not survive autoclaving. They use ethylene
>oxide,
>> >> >but that might be a bit out of reach for hobbyists.
>> >> >
>> >> >-Rob
>> >> >
>> >> >On Tue, Apr 24, 2012 at 10:37 AM, Zebedee Boy
><zebedeeboy@hotmail.com>
>> >> >wrote:
>> >> >> Has anyone come up with a way to recycle the plastic plates
>used for
>> >> >agar
>> >> >> plates? Can I just flip the used agar out and heat sterilise
>before
>> >> >using
>> >> >> again?
>> >> >>
>> >> >> Zeb
>> >> >>
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>> >> >
>> >> >
>> >> >--
>> >> >Rob O'Callahan
>> >> >M.S. Civil & Environmental Engineering 2012
>> >> >George R. Brown School of Engineering | Rice University
>> >> >E: rpo1@rice.edu<mailto:rpo1@rice.edu>| P: 847.641.1285
>> >> >
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>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
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