[DIYbio] Re: facing problem with pcr gel results

Taq should be able to amplify that just fine, I do it regularly in the lab.

Couple of questions:

Concentration of genomic DNA, Taq can be temperamental with too much template?

PCR protocol, temperatures times etc?

Tm of Primers?

Salt recipe?

John
On Tuesday, May 15, 2012 6:53:58 AM UTC-4, jizzs wrote:

i have been running pcr gel for a 1.3kb gene but getting nothing except long smears with no primer dimers at end .

i dnt know what is the problem ......

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