Hi
Unfortunatlly homologous recombination is a very difficult process to achieve. Here you can read some articles about it:
http://www.unige.ch/sciences/biologie/plantsciences/grpaszkowski/Paszkowski_lab/Publications_files/Current%20Opinion%20in%20Plant%20Biology%202003%20Hanin.pdf
http://www.pnas.org/content/96/13/7398.full.pdf
http://www.botanik2.uni-karlsruhe.de/download/pmb02r.pdf
The most common method for plant transformation usually consists in the use of Agrobacterium tunisienses. It is a very simple method to transform Arabidopsis using flower dipping. However for other plants (like crops) this method has to be combined with plant cell cultures transformation or tissue transformation followed by regeneration in both cases to obtain the transformant. Other methods are: gene gun, electroporation or Transcription Activator-Like Effector Nucleases (TALENs). All methods are based in random insertion of the DNA sequence, only the last one allow to target it to a specific genome region.
I agree with shamrock, you have to be very careful with this, because GMOs have hard laws in them, especially in Europe. I don't know but I think that you should get first a license to work with GMOs out of research facilities. The other possibility is to do the work with cooperation with a University lab. I am only saying this because the main reason taking me so much time to start to do something related with DIYBio is related with the laws in my country and all the licenses that need to be obtained.
I hope that this could help
Terça-feira, 1 de Maio de 2012 19:35:49 UTC+1, Mega escreveu:
Hey guys,
I've learned very much of the function of DNA and plasmids here...
What I yet don't know:
What are the methods to get DNA into the genome?
Could you give me some keywords so that I can do a google research on that?
(I know there are e.g. Agrobacterium transformation and bacteriophage, but what about gene gun etc. ? )
Am I right, that you can insert DNA of interest into a short gene sequence of the host, and then put it into the host?
So it will make homologuos recombination with the strand and the insert is in the genome??
Thanks!
Terça-feira, 1 de Maio de 2012 19:35:49 UTC+1, Mega escreveu:
Hey guys,
I've learned very much of the function of DNA and plasmids here...
What I yet don't know:
What are the methods to get DNA into the genome?
Could you give me some keywords so that I can do a google research on that?
(I know there are e.g. Agrobacterium transformation and bacteriophage, but what about gene gun etc. ? )
Am I right, that you can insert DNA of interest into a short gene sequence of the host, and then put it into the host?
So it will make homologuos recombination with the strand and the insert is in the genome??
Thanks!
Terça-feira, 1 de Maio de 2012 19:35:49 UTC+1, Mega escreveu:
Hey guys,
I've learned very much of the function of DNA and plasmids here...
What I yet don't know:
What are the methods to get DNA into the genome?
Could you give me some keywords so that I can do a google research on that?
(I know there are e.g. Agrobacterium transformation and bacteriophage, but what about gene gun etc. ? )
Am I right, that you can insert DNA of interest into a short gene sequence of the host, and then put it into the host?
So it will make homologuos recombination with the strand and the insert is in the genome??
Thanks!
Terça-feira, 1 de Maio de 2012 19:35:49 UTC+1, Mega escreveu:
Hey guys,
I've learned very much of the function of DNA and plasmids here...
What I yet don't know:
What are the methods to get DNA into the genome?
Could you give me some keywords so that I can do a google research on that?
(I know there are e.g. Agrobacterium transformation and bacteriophage, but what about gene gun etc. ? )
Am I right, that you can insert DNA of interest into a short gene sequence of the host, and then put it into the host?
So it will make homologuos recombination with the strand and the insert is in the genome??
Thanks!
Terça-feira, 1 de Maio de 2012 19:35:49 UTC+1, Mega escreveu:
Hey guys,--
I've learned very much of the function of DNA and plasmids here...
What I yet don't know:
What are the methods to get DNA into the genome?
Could you give me some keywords so that I can do a google research on that?
(I know there are e.g. Agrobacterium transformation and bacteriophage, but what about gene gun etc. ? )
Am I right, that you can insert DNA of interest into a short gene sequence of the host, and then put it into the host?
So it will make homologuos recombination with the strand and the insert is in the genome??
Thanks!
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