Re: [DIYbio] facing problem with pcr gel results

its dictyostellium genome... finally resuspended in 1:5 TE buffer.

On Wednesday, May 16, 2012 4:53:11 AM UTC+5:30, phillyj wrote:

I don't know where your gDNA is coming from, but maybe you got some DNAse in it? What is this sample from? How was it collected/stored?

On Tue, May 15, 2012 at 1:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:

can you label the gel lanes, other than the MW ladder I'm not sure what to make of anything

On Tue, May 15, 2012 at 6:53 AM, jizzs <jitesh.green@gmail.com> wrote:

i have been running pcr gel for a 1.3kb gene but getting nothing except long smears with no primer dimers at end .

i dnt know what is the problem ......

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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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