Re: [DIYbio] How to get surface proteins from one bacteria to another? Can somebody help?

Any living organism injected, will cause
an immune response, which may go terribly wrong: e.g. sepsis
Therefore you would have to kill the bacteria anyways
before injection.

It would be more suitable to purify the H and O antigens
from a bacterial culture and inject them with some PAMP.
But I guess, these proteins have a high variation,
else the immune system wouldn't have a problem with them.

And:
You could accidently produce e.g. a pathogenic Bacillus
subtilis by these means.
Also you would probably need to handle a pathogenic Salmonella.
Therefore your undertaking would require an
official permission e.g. in Germany (GenTG §8).

Cheers, Matthias


Am Freitag, den 25.05.2012, 17:20 -0700 schrieb Nils Reither:
> Hi
> I wonder if it is possible to get the genes from the O and H antigen
> proteins in Salmonalla bacteria transferred to more harmless bacteria
> such as Bacillus subtilus or Lactobacillus.
> This way a risk free immunisation would be possible.
>
>
> Cheers
> Nils Reither, veterinarian




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