I mean it doesn't have to be pure.
It must not be degraded by DNases. But it should work for a transformation.
distilled white vinegar? Have to look about that.
potassium chloride, sodium chloride and diatomaceous eart ?
What do I need these for? Do you have a protocol for the whole miniprep? That would be really great!!
2012/5/27 Cory Tobin <cory.tobin@gmail.com>
> I was wondering if I could do a dirty miniprep without EDTA.It will work without EDTA.
Make sure the vinegar is distilled white vinegar. Also you'll need
> What I have: weak centrifuge, SLS (sodium lauryl sulfate), NaOH, vinegar,
> ethanol (I have access to both wodka and pure ethanol), water.
potassium chloride, sodium chloride and diatomaceous earth.
I'm not sure what you mean by 60%. But I've been able to get 200ng/uL
> Can you do a mp with this limited resources?
> What I want: Some ~60 to 70 % plasmids, some remaining proteins, RNA, mabe
> some fragments of chromosomal DNA.
using only these reagents.
-cory
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