Re: [DIYbio] Miniprep without EDTA

> I was wondering if I could do a dirty miniprep without EDTA.

It will work without EDTA.


> What I have: weak centrifuge, SLS (sodium lauryl sulfate), NaOH, vinegar,
> ethanol (I have access to both wodka and pure ethanol), water.

Make sure the vinegar is distilled white vinegar. Also you'll need
potassium chloride, sodium chloride and diatomaceous earth.


> Can you do a mp with this limited resources?
> What I want: Some ~60 to 70 % plasmids, some remaining proteins, RNA, mabe
> some fragments of chromosomal DNA.

I'm not sure what you mean by 60%. But I've been able to get 200ng/uL
using only these reagents.



-cory

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