Re: [DIYbio] Miniprep without EDTA

> Is the KCl relly necessary? Can't be replaced by NaCl?

You need a potassium ion. In the commercial miniprep kits (ie.
Qiagen) they use potassium acetate, but you can only get that from
chemical suppliers. You can get KCl at the hardware store. It is
used in water softener tanks. I actually found it at a grocery store.


> What about Ethanol instead of Isopropanol?
> Feasible?

Yes, I used isopropanol because I thought people would have easy
access to 100% isopropanol whereas 100% ethanol is more difficult to
find.


> (lye is NaOH, right? )

Yes.


-cory

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